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lamp1 halo  (Addgene inc)


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    Structured Review

    Addgene inc lamp1 halo
    Lamp1 Halo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamp1+halo/pEBG-TFII-I-delta+N90+(Plasmid+%2322165)/pmc12335601-447-10-12
    Average 93 stars, based on 3 article reviews
    lamp1 halo - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
    Article Snippet: .. The following plasmids were used: mito-DsRed2 (a gift from T. Schwarz, Harvard Medical School), EGFP–FMRP (gift from G. Bassell, Emory University), GFP-POLG2 (gift from W. Copeland, NIH), Halo–FMRP (subcloned from EGFP–FMRP into pFN21A-HaloTag-CMV vector from Promega), COX8A–BFP (Goldsmith et al.49), LAMP1–Halo (Gallagher and Holzbaur50), GFP–MFF (Addgene, 49153), GFP-RAB7 (Addgene, 12605), GFP–RAB7 T22N (Addgene, 12660), RFP–RAB5 (Addgene, 14437), EGFP– DRP1 (subcloned from pcDNA 3.1-Drp1 (Addgene, 34706) into pEGFP-C1 vector), pCRISPRia-vs2 (Addgene, 84832), PGK 4xMito-mEmerald (Addgene, 200430), pUbC-OsTIR1-myc-IRES-scFv-sfGFP (Addgene, 84563), AID–SunTag–MFF (subcloned from GFP–MFF, MFF cDNA clone (Transomic BC000797) and pUbC-FLAG-24xSuntagV4-oxEBFPAID-baUTR1-24xMS2V5-Wpre (Addgene, 84561) into pEGFP-N1 vector with EGFP removed), EGFP–TWINKLE (subcloned from TWINKLE– APEX2-V5 (Addgene, 129705) into pEGFP-N1 vector), EGFP–TFAM (subcloned from pCellFree_G03 TFAM into pEGFP-N1 vector) and Halo–Stop (Cason et al. 51). ..

    Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
    Article Snippet: .. The following plasmids were used: mito-DsRed2 (a gift from T. Schwarz, Harvard Medical School), EGFP–FMRP (gift from G. Bassell, Emory University), GFP-POLG2 (gift from W. Copeland, NIH), Halo–FMRP (subcloned from EGFP–FMRP into pFN21A-HaloTag-CMV vector from Promega), COX8A–BFP (Goldsmith et al. ), LAMP1–Halo (Gallagher and Holzbaur ), GFP–MFF (Addgene, 49153), GFP-RAB7 (Addgene, 12605), GFP–RAB7 T22N (Addgene, 12660), RFP–RAB5 (Addgene, 14437), EGFP–DRP1 (subcloned from pcDNA 3.1-Drp1 (Addgene, 34706) into pEGFP-C1 vector), pCRISPRia-vs2 (Addgene, 84832), PGK 4xMito-mEmerald (Addgene, 200430), pUbC-OsTIR1-myc-IRES-scFv-sfGFP (Addgene, 84563), AID–SunTag–MFF (subcloned from GFP–MFF, MFF cDNA clone (Transomic BC000797) and pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre (Addgene, 84561) into pEGFP-N1 vector with EGFP removed), EGFP–TWINKLE (subcloned from TWINKLE–APEX2-V5 (Addgene, 129705) into pEGFP-N1 vector), EGFP–TFAM (subcloned from pCellFree_G03 TFAM into pEGFP-N1 vector) and Halo–Stop (Cason et al. ). ..

    Transfection:

    Article Title: Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy
    Article Snippet: .. The following plasmids were used for transfections: EGFP-Rubicon (RRID: Addgene_221659), LAMP1-Halo (RRID: Addgene_221655), mCherry-LC3 (RRID: Addgene_221656), mCherry-Rubicon WT (RRID: Addgene_221657), mCherry-Rubicon CGHL (RRID: Addgene_221658) and EGFP-Rab7A (RRID: Addgene_28047), EGFP-N1 (RRID: Addgene_6085-1). .. HeLa cells were transfected with 1.5 μg of untagged human Parkin (RRID: Addgene_187897) using Lipofectamine 2000 for 18–24 hrs prior to treatment.

    Article Title: Mitochondrial damage triggers concerted degradation of negative regulators of neuronal autophagy
    Article Snippet: .. The following plasmids were used for transfections: EGFP-Rubicon (RRID: Addgene 221657), LAMP1-Halo (RRID: Addgene 221655), mCherry-LC3 (RRID: Addgene 221656), mCherry-Rubicon WT (RRID: Addgene 221658), mCherry-Rubicon CGHL (RRID: Addgene 221659) and EGFP-Rab7A (RRID: Addgene 28047), EGFP-N1 (RRID: Addgene 6085-1). .. HeLa cells were transfected with 1.5 μg of untagged human Parkin (RRID: Addgene 187897) using Lipofectamine 2000 for 18-24 hrs prior to treatment.



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    HUVEC transfected with either mRFP-Halo ( A ) or <t>mRFP-Halo-LAMP1</t> ( B ) were incubated with 1 µM MaPCa-656 low for 2 h at 37 °C and subjected to live cell microscopy. Note that not all mRFP-Halo-LAMP1 positive lysosomes show enhanced MaPCa-656 low fluorescence. Shown are confocal microscopy images displaying maximum intensity projections of z-stacks. Note the cytosolic (A) or lysosomal recruitment (B) of the Ca 2+ indicator MaPCa-656 low . Scale bars: 10 μm.
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    paper rrid addgene 190168 lamp1 halo  (Addgene inc)
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    HUVEC transfected with either mRFP-Halo ( A ) or <t>mRFP-Halo-LAMP1</t> ( B ) were incubated with 1 µM MaPCa-656 low for 2 h at 37 °C and subjected to live cell microscopy. Note that not all mRFP-Halo-LAMP1 positive lysosomes show enhanced MaPCa-656 low fluorescence. Shown are confocal microscopy images displaying maximum intensity projections of z-stacks. Note the cytosolic (A) or lysosomal recruitment (B) of the Ca 2+ indicator MaPCa-656 low . Scale bars: 10 μm.
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    Image Search Results


    HUVEC transfected with either mRFP-Halo ( A ) or mRFP-Halo-LAMP1 ( B ) were incubated with 1 µM MaPCa-656 low for 2 h at 37 °C and subjected to live cell microscopy. Note that not all mRFP-Halo-LAMP1 positive lysosomes show enhanced MaPCa-656 low fluorescence. Shown are confocal microscopy images displaying maximum intensity projections of z-stacks. Note the cytosolic (A) or lysosomal recruitment (B) of the Ca 2+ indicator MaPCa-656 low . Scale bars: 10 μm.

    Journal: PLOS ONE

    Article Title: Chemigenetic Ca 2+ indicators report elevated Ca 2+ levels in endothelial Weibel-Palade bodies

    doi: 10.1371/journal.pone.0316854

    Figure Lengend Snippet: HUVEC transfected with either mRFP-Halo ( A ) or mRFP-Halo-LAMP1 ( B ) were incubated with 1 µM MaPCa-656 low for 2 h at 37 °C and subjected to live cell microscopy. Note that not all mRFP-Halo-LAMP1 positive lysosomes show enhanced MaPCa-656 low fluorescence. Shown are confocal microscopy images displaying maximum intensity projections of z-stacks. Note the cytosolic (A) or lysosomal recruitment (B) of the Ca 2+ indicator MaPCa-656 low . Scale bars: 10 μm.

    Article Snippet: Rev VWF-mRFP backbone 5´-TTGTACAGGGCGCCGGTG . mRFP-Halo-LAMP1 was obtained by exchanging RpHLuorin2 in LAMP1-RpHLuorin2 (Addgene #171720) with mRFP-Halo amplified from VWF-mRFP-Halo using NEBuilder Hifi DNA Assembly.

    Techniques: Transfection, Incubation, Microscopy, Fluorescence, Confocal Microscopy