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lamp1 halo  (Addgene inc)


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    Structured Review

    Addgene inc lamp1 halo
    Lamp1 Halo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamp1+halo/pmc12335601-447-10-12?v=Addgene+inc
    Average 93 stars, based on 3 article reviews
    lamp1 halo - by Bioz Stars, 2026-08
    93/100 stars

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    HUVEC transfected with either mRFP-Halo ( A ) or <t>mRFP-Halo-LAMP1</t> ( B ) were incubated with 1 µM MaPCa-656 low for 2 h at 37 °C and subjected to live cell microscopy. Note that not all mRFP-Halo-LAMP1 positive lysosomes show enhanced MaPCa-656 low fluorescence. Shown are confocal microscopy images displaying maximum intensity projections of z-stacks. Note the cytosolic (A) or lysosomal recruitment (B) of the Ca 2+ indicator MaPCa-656 low . Scale bars: 10 μm.
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    Image Search Results


    HUVEC transfected with either mRFP-Halo ( A ) or mRFP-Halo-LAMP1 ( B ) were incubated with 1 µM MaPCa-656 low for 2 h at 37 °C and subjected to live cell microscopy. Note that not all mRFP-Halo-LAMP1 positive lysosomes show enhanced MaPCa-656 low fluorescence. Shown are confocal microscopy images displaying maximum intensity projections of z-stacks. Note the cytosolic (A) or lysosomal recruitment (B) of the Ca 2+ indicator MaPCa-656 low . Scale bars: 10 μm.

    Journal: PLOS ONE

    Article Title: Chemigenetic Ca 2+ indicators report elevated Ca 2+ levels in endothelial Weibel-Palade bodies

    doi: 10.1371/journal.pone.0316854

    Figure Lengend Snippet: HUVEC transfected with either mRFP-Halo ( A ) or mRFP-Halo-LAMP1 ( B ) were incubated with 1 µM MaPCa-656 low for 2 h at 37 °C and subjected to live cell microscopy. Note that not all mRFP-Halo-LAMP1 positive lysosomes show enhanced MaPCa-656 low fluorescence. Shown are confocal microscopy images displaying maximum intensity projections of z-stacks. Note the cytosolic (A) or lysosomal recruitment (B) of the Ca 2+ indicator MaPCa-656 low . Scale bars: 10 μm.

    Article Snippet: Rev VWF-mRFP backbone 5´-TTGTACAGGGCGCCGGTG . mRFP-Halo-LAMP1 was obtained by exchanging RpHLuorin2 in LAMP1-RpHLuorin2 (Addgene #171720) with mRFP-Halo amplified from VWF-mRFP-Halo using NEBuilder Hifi DNA Assembly.

    Techniques: Transfection, Incubation, Microscopy, Fluorescence, Confocal Microscopy